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viability dye fixable viability dye-efluor780  (Thermo Fisher)


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    Structured Review

    Thermo Fisher viability dye fixable viability dye-efluor780
    Viability Dye Fixable Viability Dye Efluor780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixable+viability+dye-efluor780/efluor780+viability+dye+65+0865/pmc12275383-63-5-10
    Average 90 stars, based on 1 article reviews
    viability dye fixable viability dye-efluor780 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Staining:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Immunohistochemistry:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Immunohistochemistry-Frozen:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Western Blot:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Binding Assay:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Flow Cytometry:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Immunofluorescence:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    FACS:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.

    Blocking Assay:

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation.
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and Fc blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Bispecific antibodies promote natural killer cell-mediated elimination of HIV-1 reservoir cells.
    Article Snippet: 317321, 302233 and 304607, respectively), 6× His tag-AF488 and Fixable Viability Dye-eFluor780 (Thermo Fisher Scientific, catalog nos, MA1-135-A488 and 65-0865-14, respectively).

    Article Title: NK- and T-cell granzyme B and K expression correlates with age, CMV infection and influenza vaccine-induced antibody titres in older adults
    Article Snippet: Following thawing, PBMCs were resuspended in flow cytometry staining buffer (PBS, 2% FBS, 2 mM EDTA, .01% NaN3) and incubated first with Fixable Viability Dye (FvD)-eFluor780 (eBioscience, CA, United States) and 10% heat-inactivated human AB serum (Sigma-Aldrich, MO, United States) for 15 min at 4°C.

    Article Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
    Article Snippet: List of Antibodies Target(-Conjugate) Clone Catalog Number Supplier Dilution Method CD34-eFluor660 RAM34 50-0341-82 Thermo Fisher Scientific 1/100 FC CD49f/ITGa6-PE GoH3 12-0495-82 Thermo Fisher Scientific 1/500 FC CD45-PE.Cy7 30-F11 103114 BioLegend 1/50 FC CD31-PE.Cy7 390 561410 BD Pharmingen 1/50 FC DAPI D1306 Thermo Fisher Scientific 0.1 mg/ml FC eBioscience Fixable Viability Dye-eFluor780 Invitrogen 1/10000 FC Propidium Iodide P3566 Invitrogen 1/10 FC gH2AX(ph-Ser139) 20E3 9718 Cell Signaling Technologies 1/50 IHC-FFPE CD45 30-F11 553076 BD Biosciences 1/100 IHC-FFPE HMGB1 EPR3507 ab79823 Abcam 1/300 IHC-FFPE LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IHC-FFPE NIPP1 HPA027452 Sigma 1/100 IHC-FFPE CD34 RAM34 14-0341-82 Thermo Fisher Scientific 1/50 IHC-Fr FoxP3 D6O8R 12653S Cell Signaling Technologies 1/100 IHC-Fr Ki67 SP6 ab16667 Abcam 1/250 IHC-Fr gH2AX(ph-Ser139) JBW301 05-636 Millipore 1/200; 1/100 IF 53bp1 NB100-304 Novus Biologicals 1/1000 IF HMGB1 EPR3507 ab79823 Abcam 1/250 IF LaminB1 EPR22165-121 ab229025 Abcam 1/1000 IF NIPP1 HPA027452 Sigma 1/100 IF p21 orb31546 Bio-Orbyt 1/100 IF DAPI 1/10,000 IF b-actin AC-15 sc-69879 Santa Cruz 1/2000; 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Laminb1 EPR22165-121 ab229025 Abcam 1/1000 WB NIPP1 HPA027452 Sigma 1/1000 WB p16 EPR20418 ab2111542 Abcam 1/1000 WB p19 5-C3-1 NB200-174 Novus Biologicals 1/1000 WB p21 12D1 2947S Cell Signaling Technologies 1/1000 WB p53 9282S Cell Signaling Technologies 1/1000 WB TATA binding protein ab51841 Abcam 1/5000 WB y-Tubulin ab11317 Abcam 1/2000 WB Abbreviations: FC, flow cytometry; FFPE, formalin-fixed paraffin-embedded; Fr, frozen cryosection; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot. www.jidonline.org 14.e11 Supplementary Table S5.

    Article Title: Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukemia.
    Article Snippet: Barcoded samples were washed, pooled and labeled with Fixable Viability Dye-eFluor780 (Affymetrix eBioscience) during 15 min at 4°C.

    Article Title: Microbiome biomarkers immunotherapy responsiveness: diagnostic, prognostic and therapeutic uses thereof
    Article Snippet: Tumor cell suspensions were subsequently stained with CD3-AX700 (Ebioscience, 17A2), CD8α-Pacific Blue (Biolegend, 53-6.7), CD4-BV711 (Biolegend, RM4-5), CD44-FITC (BD, IM7), and Fixable Viability Dye-eFluor780 (Ebioscience).

    Article Title: Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation
    Article Snippet: Briefly, cells were washed with FACS buffer (RPMI-1640 + 1% FBS + 1X DPBS + 5 mM EDTA), followed by staining with fixable viability Dye-eFluor780 (Thermo Scientific, 65-0865-18) for 10 minutes at 4 °C and F c blocking (Thermo Scientific, 16-0161-82 for mice and BioLegend 422302 for humans) for 10 minutes at room temperatures.

    Article Title: Nanomaterials trigger functional responses in primary human immune cells
    Article Snippet: After incubation, the cells were washed in PBS and non-viable cells were stained with Fixable Viability Dye-eFluor780 (eBioscience #65-0865-14) for 15 minutes at room temperature in the dark.



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